Which of the following is one of the major interstates that runs through West Virginia?

Interstate 64 is indeed one of the major interstates that runs through West Virginia. This interstate spans from Virginia to Kentucky and is a critical route for both commerce and travel in the region. It passes through several key cities in West Virginia, including Huntington and Charleston, facilitating transportation and connectivity for residents and visitors alike.

The other routes listed do not significantly traverse West Virginia. For example, while Interstate 70 does pass through West Virginia briefly, its primary corridor is in Ohio and Pennsylvania. Interstate 80 primarily travels across Pennsylvania and does not enter West Virginia at all. Similarly, while Interstate 76 connects parts of Pennsylvania, it does not extend into West Virginia. Thus, Interstate 64 stands out as a major route within the state, making it the correct choice for this question.